II. Measuring protein proximities on CAR T cell surfaces

Using Molecular Pixelation-based spatial proteomics, we analyze protein proximities at single-cell resolution across 8,000+ CAR T cells. In chronically stimulated cells, roughly half of the 76 surface markers show increased polarization, with notable changes in adhesion-associated proteins in the immune synapse.

These spatial rearrangement measurements reveal phenotypic information that’s invisible with protein expression alone, offering a new look into exhaustion at the T cell surface.

Research papers

(1) Cesnik, A.†; Takacsi-Nagy, O.; Roth, T. L.; Le, T.; Satpathy, A. T.; Lundberg, E.† ``Molecular pixelation of the CAR T cell surface proteome.’’ bioRxiv 2026, 2026.01.30.702970. †Co-corresponding https://doi.org/10.64898/2026.01.30.702970