Area 3: Discerning proteoform function with spatial proteomics.
Spatial variations in protein expression within cells are widespread and can allow proteins to have multiple functions. We will garner experimental evidence of such functional proteoform variation by collecting and integrating both microscopy and mass spectrometry based proteomic datasets for sitespecific modified proteoforms. We will combine these two data modalities to build a data-driven map of proteoform localizations, interactions, and functions at all levels of cellular organization. This will enable broad usage of these data to better understand proteoform variation in human health.
